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label cell nuclei  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc label cell nuclei
    Label Cell Nuclei, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/label+cell+nuclei/SPY555-FastAct/bio_rxiv__2025__09__28__678961-184-18-23
    Average 94 stars, based on 12 article reviews
    label cell nuclei - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Matrix viscoelasticity regulates dendritic cell migration and immune priming
    Article Snippet: For tracking collagen displacement, collagen was fluorescently labeled by incubating DC-encapsulating Nb-Col with tetrazine-iFluor 488 (AAT Bioquest, 1014) at 37 °C for 1 hour to allow the click reaction, followed by two washes with DC media. .. SiR-DNA (Cytoskeleton, CY-SC007) was added 1 day prior to imaging at a final concentration of 200 nM to label cell nuclei, and SPY555-FastAct (Cytoskeleton, CY-SC205; 1:2000) was added 1 hour prior to imaging to label actin. .. Time-lapse imaging for collagen displacement was performed using a Zeiss LSM 980 confocal microscope, with images captured every minute over a 10-minute span.

    Concentration Assay:

    Article Title: Matrix viscoelasticity regulates dendritic cell migration and immune priming
    Article Snippet: For tracking collagen displacement, collagen was fluorescently labeled by incubating DC-encapsulating Nb-Col with tetrazine-iFluor 488 (AAT Bioquest, 1014) at 37 °C for 1 hour to allow the click reaction, followed by two washes with DC media. .. SiR-DNA (Cytoskeleton, CY-SC007) was added 1 day prior to imaging at a final concentration of 200 nM to label cell nuclei, and SPY555-FastAct (Cytoskeleton, CY-SC205; 1:2000) was added 1 hour prior to imaging to label actin. .. Time-lapse imaging for collagen displacement was performed using a Zeiss LSM 980 confocal microscope, with images captured every minute over a 10-minute span.



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    AGO5 and AGO9 expression throughout development. Expression of ProAGO5:EGFP-AGO5 (in cyan, A, C, E, G, I, K, M, O, Q, S, U, W) and ProAGO9:EGFP-AGO9 (in cyan, B, D, F, H, J, L, N, P, R, T, V, X) in plant lines containing the stem cell reporter <t>ProCLV3:H2B-</t> mCherry (red) and lacking endogenous AGO5 or AGO9 . A, B) Meristems of 7-day-old seedlings. Cc, Dd) Meristems of 14-d-old seedlings. E, F) Inflorescence meristems of 35-day-old plants. G) FB in stage 7; asterisk marks the upper region of the gynecium; arrowheads the inner layers of future anthers. H) as G) Arrowhead marks the base of the bud; asterisk the gynecium (asterisk); section signs (§) the inner layers of future stamens. I, J) Microspore mother cells in developing anthers. K, L) Mature pollen. M, N) megaspore mother cells (arrowhead) in developing ovules. O, P) Egg cells (arrowhead) within embryo sacs. Q, R) Zygotes (arrowhead). S, T) Embryos at the globular stage. U, V) Embryos at the heart stage. W, X) Meristem of 21-d-old plants grown under a short daylight regime. Scale bars k, l = 5 μ m; A, B, D, I, J, M, N, S, T, U, V, W, X = 20 μ m; C, E, F, G, H, O, P, Q, R = 50 μ m.
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    Image Search Results


    AGO5 and AGO9 expression throughout development. Expression of ProAGO5:EGFP-AGO5 (in cyan, A, C, E, G, I, K, M, O, Q, S, U, W) and ProAGO9:EGFP-AGO9 (in cyan, B, D, F, H, J, L, N, P, R, T, V, X) in plant lines containing the stem cell reporter ProCLV3:H2B- mCherry (red) and lacking endogenous AGO5 or AGO9 . A, B) Meristems of 7-day-old seedlings. Cc, Dd) Meristems of 14-d-old seedlings. E, F) Inflorescence meristems of 35-day-old plants. G) FB in stage 7; asterisk marks the upper region of the gynecium; arrowheads the inner layers of future anthers. H) as G) Arrowhead marks the base of the bud; asterisk the gynecium (asterisk); section signs (§) the inner layers of future stamens. I, J) Microspore mother cells in developing anthers. K, L) Mature pollen. M, N) megaspore mother cells (arrowhead) in developing ovules. O, P) Egg cells (arrowhead) within embryo sacs. Q, R) Zygotes (arrowhead). S, T) Embryos at the globular stage. U, V) Embryos at the heart stage. W, X) Meristem of 21-d-old plants grown under a short daylight regime. Scale bars k, l = 5 μ m; A, B, D, I, J, M, N, S, T, U, V, W, X = 20 μ m; C, E, F, G, H, O, P, Q, R = 50 μ m.

    Journal: The Plant Cell

    Article Title: Two ARGONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis

    doi: 10.1093/plcell/koad295

    Figure Lengend Snippet: AGO5 and AGO9 expression throughout development. Expression of ProAGO5:EGFP-AGO5 (in cyan, A, C, E, G, I, K, M, O, Q, S, U, W) and ProAGO9:EGFP-AGO9 (in cyan, B, D, F, H, J, L, N, P, R, T, V, X) in plant lines containing the stem cell reporter ProCLV3:H2B- mCherry (red) and lacking endogenous AGO5 or AGO9 . A, B) Meristems of 7-day-old seedlings. Cc, Dd) Meristems of 14-d-old seedlings. E, F) Inflorescence meristems of 35-day-old plants. G) FB in stage 7; asterisk marks the upper region of the gynecium; arrowheads the inner layers of future anthers. H) as G) Arrowhead marks the base of the bud; asterisk the gynecium (asterisk); section signs (§) the inner layers of future stamens. I, J) Microspore mother cells in developing anthers. K, L) Mature pollen. M, N) megaspore mother cells (arrowhead) in developing ovules. O, P) Egg cells (arrowhead) within embryo sacs. Q, R) Zygotes (arrowhead). S, T) Embryos at the globular stage. U, V) Embryos at the heart stage. W, X) Meristem of 21-d-old plants grown under a short daylight regime. Scale bars k, l = 5 μ m; A, B, D, I, J, M, N, S, T, U, V, W, X = 20 μ m; C, E, F, G, H, O, P, Q, R = 50 μ m.

    Article Snippet: Segmentation and counting of H2B-mCherry-labeled stem cell nuclei were computed with Imaris 9.5.0 software.

    Techniques: Expressing